Efficacy of an Adjuvanted Middle East Respiratory Syndrome Coronavirus Spike Protein Vaccine in Dromedary Camels and Alpacas
Identifieur interne : 000605 ( Main/Exploration ); précédent : 000604; suivant : 000606Efficacy of an Adjuvanted Middle East Respiratory Syndrome Coronavirus Spike Protein Vaccine in Dromedary Camels and Alpacas
Auteurs : Danielle R. Adney ; Lingshu Wang ; Neeltje Van Doremalen ; Wei Shi ; Yi Zhang ; Wing-Pui Kong ; Megan R. Miller ; Trenton Bushmaker ; Dana Scott ; Emmie De Wit ; Kayvon Modjarrad ; Nikolai Petrovsky ; Barney S. Graham ; Richard A. Bowen ; Vincent J. MunsterSource :
- Viruses [ 1999-4915 ] ; 2019.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques (administration et posologie), Animaux, Anticorps antiviraux (sang), Anticorps neutralisants (sang), Camélidés du Nouveau Monde (immunologie), Chameaux (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Excrétion virale, Femelle, Glycoprotéine de spicule des coronavirus (immunologie), Immunité humorale, Infections à coronavirus (), Infections à coronavirus (médecine vétérinaire), Mâle, Vaccins antiviraux (immunologie).
- MESH :
- administration et posologie : Adjuvants immunologiques.
- immunologie : Camélidés du Nouveau Monde, Chameaux, Glycoprotéine de spicule des coronavirus, Vaccins antiviraux.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- médecine vétérinaire : Infections à coronavirus.
- sang : Anticorps antiviraux, Anticorps neutralisants.
- Animaux, Excrétion virale, Femelle, Immunité humorale, Infections à coronavirus, Mâle.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (administration & dosage), Animals, Antibodies, Neutralizing (blood), Antibodies, Viral (blood), Camelids, New World (immunology), Camelus (immunology), Coronavirus Infections (prevention & control), Coronavirus Infections (veterinary), Female, Immunity, Humoral, Male, Middle East Respiratory Syndrome Coronavirus (isolation & purification), Spike Glycoprotein, Coronavirus (immunology), Viral Vaccines (immunology), Virus Shedding.
- MESH :
- chemical , administration & dosage : Adjuvants, Immunologic.
- chemical , blood : Antibodies, Neutralizing, Antibodies, Viral.
- immunology : Camelids, New World, Camelus, Spike Glycoprotein, Coronavirus, Viral Vaccines.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- prevention & control : Coronavirus Infections.
- veterinary : Coronavirus Infections.
- Animals, Female, Immunity, Humoral, Male, Virus Shedding.
Abstract
MERS-CoV is present in dromedary camels throughout the Middle East and Africa. Dromedary camels are the primary zoonotic reservoir for human infections. Interruption of the zoonotic transmission chain from camels to humans, therefore, may be an effective strategy to control the ongoing MERS-CoV outbreak. Here we show that vaccination with an adjuvanted MERS-CoV Spike protein subunit vaccine confers complete protection from MERS-CoV disease in alpaca and results in reduced and delayed viral shedding in the upper airways of dromedary camels. Protection in alpaca correlates with high serum neutralizing antibody titers. Lower titers of serum neutralizing antibodies correlate with delayed and significantly reduced shedding in the nasal turbinates of dromedary camels. Together, these data indicate that induction of robust neutralizing humoral immune responses by vaccination of naïve animals reduces shedding that potentially could diminish the risk of zoonotic transmission.
Url:
DOI: 10.3390/v11030212
PubMed: 30832356
PubMed Central: 6466352
Affiliations:
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Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Efficacy of an Adjuvanted Middle East Respiratory Syndrome Coronavirus Spike Protein Vaccine in Dromedary Camels and Alpacas</title><author><name sortKey="Adney, Danielle R" sort="Adney, Danielle R" uniqKey="Adney D" first="Danielle R." last="Adney">Danielle R. Adney</name><affiliation><nlm:aff id="af1-viruses-11-00212">Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80521, USA;<email>Danielle.Adney@colostate.edu</email></nlm:aff></affiliation></author><author><name sortKey="Wang, Lingshu" sort="Wang, Lingshu" uniqKey="Wang L" first="Lingshu" last="Wang">Lingshu Wang</name><affiliation><nlm:aff id="af2-viruses-11-00212">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892, USA;<email>wangling@niaid.nih.gov</email> (L.W.);<email>shiw@mail.nih.gov</email> (W.S.);<email>yi.zhang3@nih.gov</email> (Y.Z.);<email>wkong@mail.nih.gov</email> (W.-P.K.);<email>bgraham@mail.nih.gov</email> (B.S.G.)</nlm:aff></affiliation></author><author><name sortKey="Van Doremalen, Neeltje" sort="Van Doremalen, Neeltje" uniqKey="Van Doremalen N" first="Neeltje" last="Van Doremalen">Neeltje Van Doremalen</name><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author><author><name sortKey="Shi, Wei" sort="Shi, Wei" uniqKey="Shi W" first="Wei" last="Shi">Wei Shi</name><affiliation><nlm:aff id="af2-viruses-11-00212">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892, USA;<email>wangling@niaid.nih.gov</email> (L.W.);<email>shiw@mail.nih.gov</email> (W.S.);<email>yi.zhang3@nih.gov</email> (Y.Z.);<email>wkong@mail.nih.gov</email> (W.-P.K.);<email>bgraham@mail.nih.gov</email> (B.S.G.)</nlm:aff></affiliation></author><author><name sortKey="Zhang, Yi" sort="Zhang, Yi" uniqKey="Zhang Y" first="Yi" last="Zhang">Yi Zhang</name><affiliation><nlm:aff id="af2-viruses-11-00212">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892, USA;<email>wangling@niaid.nih.gov</email> (L.W.);<email>shiw@mail.nih.gov</email> (W.S.);<email>yi.zhang3@nih.gov</email> (Y.Z.);<email>wkong@mail.nih.gov</email> (W.-P.K.);<email>bgraham@mail.nih.gov</email> (B.S.G.)</nlm:aff></affiliation></author><author><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name><affiliation><nlm:aff id="af2-viruses-11-00212">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892, USA;<email>wangling@niaid.nih.gov</email> (L.W.);<email>shiw@mail.nih.gov</email> (W.S.);<email>yi.zhang3@nih.gov</email> (Y.Z.);<email>wkong@mail.nih.gov</email> (W.-P.K.);<email>bgraham@mail.nih.gov</email> (B.S.G.)</nlm:aff></affiliation></author><author><name sortKey="Miller, Megan R" sort="Miller, Megan R" uniqKey="Miller M" first="Megan R." last="Miller">Megan R. Miller</name><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author><author><name sortKey="Bushmaker, Trenton" sort="Bushmaker, Trenton" uniqKey="Bushmaker T" first="Trenton" last="Bushmaker">Trenton Bushmaker</name><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author><author><name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author><author><name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author><author><name sortKey="Modjarrad, Kayvon" sort="Modjarrad, Kayvon" uniqKey="Modjarrad K" first="Kayvon" last="Modjarrad">Kayvon Modjarrad</name><affiliation><nlm:aff id="af4-viruses-11-00212">Military HIV Research, Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA;<email>kmodjarrad@hivresearch.org</email></nlm:aff></affiliation></author><author><name sortKey="Petrovsky, Nikolai" sort="Petrovsky, Nikolai" uniqKey="Petrovsky N" first="Nikolai" last="Petrovsky">Nikolai Petrovsky</name><affiliation><nlm:aff id="af5-viruses-11-00212">Flinders University and Vaxine Pty Ltd, Flinders Medical Centre, Bedford Park, SA 5042, Australia;<email>nikolai.petrovsky@flinders.edu.au</email></nlm:aff></affiliation></author><author><name sortKey="Graham, Barney S" sort="Graham, Barney S" uniqKey="Graham B" first="Barney S." last="Graham">Barney S. 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Munster</name><affiliation><nlm:aff id="af1-viruses-11-00212">Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80521, USA;<email>Danielle.Adney@colostate.edu</email></nlm:aff></affiliation><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">30832356</idno><idno type="pmc">6466352</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466352</idno><idno type="RBID">PMC:6466352</idno><idno type="doi">10.3390/v11030212</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">001219</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001219</idno><idno type="wicri:Area/Pmc/Curation">001219</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001219</idno><idno type="wicri:Area/Pmc/Checkpoint">000332</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000332</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:30832356</idno><idno type="wicri:Area/PubMed/Corpus">000621</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000621</idno><idno type="wicri:Area/PubMed/Curation">000621</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000621</idno><idno type="wicri:Area/PubMed/Checkpoint">000591</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000591</idno><idno type="wicri:Area/Ncbi/Merge">002135</idno><idno type="wicri:Area/Ncbi/Curation">002135</idno><idno type="wicri:Area/Ncbi/Checkpoint">002135</idno><idno type="wicri:Area/Main/Merge">000608</idno><idno type="wicri:Area/Main/Curation">000605</idno><idno type="wicri:Area/Main/Exploration">000605</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Efficacy of an Adjuvanted Middle East Respiratory Syndrome Coronavirus Spike Protein Vaccine in Dromedary Camels and Alpacas</title><author><name sortKey="Adney, Danielle R" sort="Adney, Danielle R" uniqKey="Adney D" first="Danielle R." last="Adney">Danielle R. Adney</name><affiliation><nlm:aff id="af1-viruses-11-00212">Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80521, USA;<email>Danielle.Adney@colostate.edu</email></nlm:aff></affiliation></author><author><name sortKey="Wang, Lingshu" sort="Wang, Lingshu" uniqKey="Wang L" first="Lingshu" last="Wang">Lingshu Wang</name><affiliation><nlm:aff id="af2-viruses-11-00212">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892, USA;<email>wangling@niaid.nih.gov</email> (L.W.);<email>shiw@mail.nih.gov</email> (W.S.);<email>yi.zhang3@nih.gov</email> (Y.Z.);<email>wkong@mail.nih.gov</email> (W.-P.K.);<email>bgraham@mail.nih.gov</email> (B.S.G.)</nlm:aff></affiliation></author><author><name sortKey="Van Doremalen, Neeltje" sort="Van Doremalen, Neeltje" uniqKey="Van Doremalen N" first="Neeltje" last="Van Doremalen">Neeltje Van Doremalen</name><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author><author><name sortKey="Shi, Wei" sort="Shi, Wei" uniqKey="Shi W" first="Wei" last="Shi">Wei Shi</name><affiliation><nlm:aff id="af2-viruses-11-00212">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892, USA;<email>wangling@niaid.nih.gov</email> (L.W.);<email>shiw@mail.nih.gov</email> (W.S.);<email>yi.zhang3@nih.gov</email> (Y.Z.);<email>wkong@mail.nih.gov</email> (W.-P.K.);<email>bgraham@mail.nih.gov</email> (B.S.G.)</nlm:aff></affiliation></author><author><name sortKey="Zhang, Yi" sort="Zhang, Yi" uniqKey="Zhang Y" first="Yi" last="Zhang">Yi Zhang</name><affiliation><nlm:aff id="af2-viruses-11-00212">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892, USA;<email>wangling@niaid.nih.gov</email> (L.W.);<email>shiw@mail.nih.gov</email> (W.S.);<email>yi.zhang3@nih.gov</email> (Y.Z.);<email>wkong@mail.nih.gov</email> (W.-P.K.);<email>bgraham@mail.nih.gov</email> (B.S.G.)</nlm:aff></affiliation></author><author><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name><affiliation><nlm:aff id="af2-viruses-11-00212">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892, USA;<email>wangling@niaid.nih.gov</email> (L.W.);<email>shiw@mail.nih.gov</email> (W.S.);<email>yi.zhang3@nih.gov</email> (Y.Z.);<email>wkong@mail.nih.gov</email> (W.-P.K.);<email>bgraham@mail.nih.gov</email> (B.S.G.)</nlm:aff></affiliation></author><author><name sortKey="Miller, Megan R" sort="Miller, Megan R" uniqKey="Miller M" first="Megan R." last="Miller">Megan R. Miller</name><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author><author><name sortKey="Bushmaker, Trenton" sort="Bushmaker, Trenton" uniqKey="Bushmaker T" first="Trenton" last="Bushmaker">Trenton Bushmaker</name><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author><author><name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author><author><name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author><author><name sortKey="Modjarrad, Kayvon" sort="Modjarrad, Kayvon" uniqKey="Modjarrad K" first="Kayvon" last="Modjarrad">Kayvon Modjarrad</name><affiliation><nlm:aff id="af4-viruses-11-00212">Military HIV Research, Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA;<email>kmodjarrad@hivresearch.org</email></nlm:aff></affiliation></author><author><name sortKey="Petrovsky, Nikolai" sort="Petrovsky, Nikolai" uniqKey="Petrovsky N" first="Nikolai" last="Petrovsky">Nikolai Petrovsky</name><affiliation><nlm:aff id="af5-viruses-11-00212">Flinders University and Vaxine Pty Ltd, Flinders Medical Centre, Bedford Park, SA 5042, Australia;<email>nikolai.petrovsky@flinders.edu.au</email></nlm:aff></affiliation></author><author><name sortKey="Graham, Barney S" sort="Graham, Barney S" uniqKey="Graham B" first="Barney S." last="Graham">Barney S. Graham</name><affiliation><nlm:aff id="af2-viruses-11-00212">Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892, USA;<email>wangling@niaid.nih.gov</email> (L.W.);<email>shiw@mail.nih.gov</email> (W.S.);<email>yi.zhang3@nih.gov</email> (Y.Z.);<email>wkong@mail.nih.gov</email> (W.-P.K.);<email>bgraham@mail.nih.gov</email> (B.S.G.)</nlm:aff></affiliation></author><author><name sortKey="Bowen, Richard A" sort="Bowen, Richard A" uniqKey="Bowen R" first="Richard A." last="Bowen">Richard A. Bowen</name><affiliation><nlm:aff id="af1-viruses-11-00212">Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80521, USA;<email>Danielle.Adney@colostate.edu</email></nlm:aff></affiliation></author><author><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J." last="Munster">Vincent J. Munster</name><affiliation><nlm:aff id="af1-viruses-11-00212">Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80521, USA;<email>Danielle.Adney@colostate.edu</email></nlm:aff></affiliation><affiliation><nlm:aff id="af3-viruses-11-00212">Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA;<email>neeltje.vandoremalen@nih.gov</email> (N.v.D.);<email>megan.r.miller@colostate.edu</email> (M.R.M.);<email>bushmakertj@niaid.nih.gov</email> (T.B.);<email>dana.scott@nih.gov</email> (D.S.);<email>Emmie.deWit@nih.gov</email> (E.d.W.)</nlm:aff></affiliation></author></analytic><series><title level="j">Viruses</title><idno type="eISSN">1999-4915</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (administration & dosage)</term><term>Animals</term><term>Antibodies, Neutralizing (blood)</term><term>Antibodies, Viral (blood)</term><term>Camelids, New World (immunology)</term><term>Camelus (immunology)</term><term>Coronavirus Infections (prevention & control)</term><term>Coronavirus Infections (veterinary)</term><term>Female</term><term>Immunity, Humoral</term><term>Male</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Spike Glycoprotein, Coronavirus (immunology)</term><term>Viral Vaccines (immunology)</term><term>Virus Shedding</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques (administration et posologie)</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Anticorps neutralisants (sang)</term><term>Camélidés du Nouveau Monde (immunologie)</term><term>Chameaux (immunologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Excrétion virale</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus (immunologie)</term><term>Immunité humorale</term><term>Infections à coronavirus ()</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Mâle</term><term>Vaccins antiviraux (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Adjuvants, Immunologic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Adjuvants immunologiques</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Camélidés du Nouveau Monde</term><term>Chameaux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Camelids, New World</term><term>Camelus</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Immunity, Humoral</term><term>Male</term><term>Virus Shedding</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Excrétion virale</term><term>Femelle</term><term>Immunité humorale</term><term>Infections à coronavirus</term><term>Mâle</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>MERS-CoV is present in dromedary camels throughout the Middle East and Africa. Dromedary camels are the primary zoonotic reservoir for human infections. Interruption of the zoonotic transmission chain from camels to humans, therefore, may be an effective strategy to control the ongoing MERS-CoV outbreak. Here we show that vaccination with an adjuvanted MERS-CoV Spike protein subunit vaccine confers complete protection from MERS-CoV disease in alpaca and results in reduced and delayed viral shedding in the upper airways of dromedary camels. Protection in alpaca correlates with high serum neutralizing antibody titers. Lower titers of serum neutralizing antibodies correlate with delayed and significantly reduced shedding in the nasal turbinates of dromedary camels. Together, these data indicate that induction of robust neutralizing humoral immune responses by vaccination of naïve animals reduces shedding that potentially could diminish the risk of zoonotic transmission.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alagaili, A N" uniqKey="Alagaili A">A.N. Alagaili</name></author><author><name sortKey="Briese, T" uniqKey="Briese T">T. 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Bowen</name><name sortKey="Bushmaker, Trenton" sort="Bushmaker, Trenton" uniqKey="Bushmaker T" first="Trenton" last="Bushmaker">Trenton Bushmaker</name><name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name><name sortKey="Graham, Barney S" sort="Graham, Barney S" uniqKey="Graham B" first="Barney S." last="Graham">Barney S. Graham</name><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name><name sortKey="Miller, Megan R" sort="Miller, Megan R" uniqKey="Miller M" first="Megan R." last="Miller">Megan R. Miller</name><name sortKey="Modjarrad, Kayvon" sort="Modjarrad, Kayvon" uniqKey="Modjarrad K" first="Kayvon" last="Modjarrad">Kayvon Modjarrad</name><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J." last="Munster">Vincent J. 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